| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230881 |
| Chromosome: | chromosome 2 |
| Location: | 1467583 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083850 | (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCAGGGTAATGGGGGGCCGGGAGAGCG |
| Internal bar code: | GCGCAGGAACGGGCGACCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 99.4911 |
| LEAP-Seq n confirming: | 391 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGATGCGTTGAGGGACT |
| Suggested primer 2: | CTCCAGCTCTGGGTACTTGC |