| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230887 |
| Chromosome: | chromosome_11 |
| Location: | 397065 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467580 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTGCACCAAGGCATGCACGAGATCAGCGA |
| Internal bar code: | CTTGCCTACGCGCCCCGTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 94 |
| LEAP-Seq percent confirming: | 70.4918 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACTGGGCTACAGGAGAT |
| Suggested primer 2: | ACACACACACACACACGCAC |