Insertion junction: LMJ.RY0402.230888_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230888
Chromosome:chromosome 1
Location:8002767
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g070202 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAACCGCAAGTCGTTTGCGGCGCTAGAG
Internal bar code:CCGCCGAAACGCCATGCGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:607
LEAP-Seq percent confirming:98.3122
LEAP-Seq n confirming:699
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCAATCCAGTAAACAGGC
Suggested primer 2:GCGTAAGAATTTAGCGCCTG