| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230894 |
| Chromosome: | chromosome 1 |
| Location: | 5757382 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041100 | HLM3 | (1 of 1) PTHR12197:SF75 - HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2; Histone-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGCCTGCGGCGTGTGCTGCTGCGGCTG |
| Internal bar code: | TGAGCCCAGTCGTGACTTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.1379 |
| LEAP-Seq n confirming: | 345 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCATGCACGCTTCGTATC |
| Suggested primer 2: | GGCCTCAAGAAGCGTGTAAG |