| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230899 |
| Chromosome: | chromosome 1 |
| Location: | 1740255 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009325 | (1 of 2) 1.14.11.18 - Phytanoyl-CoA dioxygenase / Phytanoyl-CoA hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTGCTCCACGCCTCCGGACCCAACACC |
| Internal bar code: | AAGTCCGGGGTAGGTTCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 99.3852 |
| LEAP-Seq n confirming: | 2748 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGTATTGTGCAAGCTGA |
| Suggested primer 2: | GATGTTGGGTGACGTGACAG |