Insertion junction: LMJ.RY0402.230908_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.230908
Chromosome:chromosome 8
Location:697910
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g360300 SDR11 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.300//1.1.1.330 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) // Very-long-chain 3-oxoacyl-CoA reductase / Very-long-chain beta-ketoacyl-CoA reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTCTGCAACTGTGCTGACGCTTCGGCA
Internal bar code:GATTTGGATCTGCGGATATTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:531
LEAP-Seq percent confirming:98.6645
LEAP-Seq n confirming:5467
LEAP-Seq n nonconfirming:74
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACAGAGAAGCCTTTGGG
Suggested primer 2:GGCTTGTGAGGTACCGTGTT