Insertion junction: LMJ.RY0402.230908_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230908
Chromosome:chromosome 8
Location:697919
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g360300 SDR11 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.300//1.1.1.330 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) // Very-long-chain 3-oxoacyl-CoA reductase / Very-long-chain beta-ketoacyl-CoA reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAAGTTCCCGACACGCCCAACCCCGCCCC
Internal bar code:AACTGGGAGGCGCGTATACCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:591
LEAP-Seq percent confirming:94.0299
LEAP-Seq n confirming:189
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACAGAGAAGCCTTTGGG
Suggested primer 2:GGCTTGTGAGGTACCGTGTT