| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230948 |
| Chromosome: | chromosome 1 |
| Location: | 2540104 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014650 | PHX2,P4H5,PFH5 | Prolyl 4-hydroxylase 5; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGATATACCCTCAGGCTTTCGCCAGC |
| Internal bar code: | TTGTTGGATCAGTAGCAGAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 98.046 |
| LEAP-Seq n confirming: | 853 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTTAGCAGCTTGCAGAC |
| Suggested primer 2: | AACCCATCCGTGTCACTAGC |