| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230954 |
| Chromosome: | chromosome 8 |
| Location: | 2319077 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370500 | (1 of 1) IPR013024//IPR017939 - Butirosin biosynthesis, BtrG-like // Gamma-glutamylcyclotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCACCGGTGCCGTCGTATGGCAGCTCT |
| Internal bar code: | GATAACGTAGTTCGATCTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 99.3327 |
| LEAP-Seq n confirming: | 8932 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGGTTTAGCAGCGAGGA |
| Suggested primer 2: | GTGGTGGTCATTTCCAAACC |