| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230956 |
| Chromosome: | chromosome 12 |
| Location: | 4087278 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517800 | (1 of 18) PF12906 - RING-variant domain (RINGv) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCACTTCAGCATCAGCTGGTGGATGCAG |
| Internal bar code: | ACGTTTCGTCAATATGCACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 96.8238 |
| LEAP-Seq n confirming: | 945 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTGTGCTACATCGTGG |
| Suggested primer 2: | TACACGTAACCTGCTCAGCG |