| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230969 |
| Chromosome: | chromosome 1 |
| Location: | 340043 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002050 | APT1 | Acid phosphatase; (1 of 1) K01078 - acid phosphatase (E3.1.3.2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGGGCGCAGACCGGCAGTGGCTCCTG |
| Internal bar code: | AGATCTGTCATGAACGGCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.7561 |
| LEAP-Seq n confirming: | 409 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTCTGTCCACCTTCTCG |
| Suggested primer 2: | TAACGATGTGCAGGCAAGAG |