Insertion junction: LMJ.RY0402.230995_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230995
Chromosome:chromosome 13
Location:2783783
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g582800 (1 of 1) 6.3.2.11 - Carnosine synthase / Carnosine synthetase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCACCACCATGAGCTCGCACAGCCACGTG
Internal bar code:CCCATCCCGGAACTGGTGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:597
LEAP-Seq percent confirming:78.5023
LEAP-Seq n confirming:2757
LEAP-Seq n nonconfirming:755
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGGACAACACCACTCCT
Suggested primer 2:GGATGTTTGGAAAAGCGGTA