| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231030 |
| Chromosome: | chromosome 16 |
| Location: | 5825881 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677920 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCACAGGCCTCTGAAGGGCTCTCAAGGG |
| Internal bar code: | AACGACATTGCGGCCGCCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 96.5217 |
| LEAP-Seq n confirming: | 444 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAATTCGTAACGGCAGG |
| Suggested primer 2: | AGTGCTGGATTGGGAACTTG |