Insertion junction: LMJ.RY0402.231038_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.231038
Chromosome:chromosome 7
Location:3912551
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g339104 (1 of 2) 3.6.3.19 - Maltose-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTTCAAGAGCTGCTGCCGGAACCACCCA
Internal bar code:ATTTCGCAGGGGCTGTCTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:598
LEAP-Seq percent confirming:98.7376
LEAP-Seq n confirming:1095
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGTTTAGTTTGGGCTGG
Suggested primer 2:GCATTACTCGTATGCGCTGA