| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231045 |
| Chromosome: | chromosome 2 |
| Location: | 3458230 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCAGGTACCAACATGGTGCCAAGATCC |
| Internal bar code: | CCTACTTTGAGCCATGTATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 403 |
| LEAP-Seq percent confirming: | 99.1684 |
| LEAP-Seq n confirming: | 477 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGCTGAGTCGCCCTAAG |
| Suggested primer 2: | GAAGCATGCAAGACAGGACA |