| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231051 |
| Chromosome: | chromosome_3 |
| Location: | 9157706 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g212305 | FAP181 | Flagellar Associated Coiled-Coil Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCTCGAGCCATACACCAAGCACCGTGCCC |
| Internal bar code: | GAAGTCCCCCCTTGCTCCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 97.402 |
| LEAP-Seq n confirming: | 47238 |
| LEAP-Seq n nonconfirming: | 1260 |
| LEAP-Seq n unique pos: | 287 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCCTATGCTGTATTGTT |
| Suggested primer 2: | GGACAAAATGGCAGAAGCAT |