Insertion junction: LMJ.RY0402.231052_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.231052
Chromosome:chromosome 6
Location:7791799
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g302450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGTGCAGACACCCGATTTGTTTGGCGGT
Internal bar code:ATTGGCTACCCGCGCGGCACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:329
LEAP-Seq percent confirming:69.6104
LEAP-Seq n confirming:536
LEAP-Seq n nonconfirming:234
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCTTTTAGCAAGGCCACA
Suggested primer 2:GACTAGGCTTGTCAAAGCGG