| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231064 |
| Chromosome: | chromosome 5 |
| Location: | 436528 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232752 | COCA1,COA1 | (1 of 1) PF08695 - Cytochrome oxidase complex assembly protein 1 (Coa1); Cytochrome oxidase complex assembly protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACCGCCGCAGCATTCAACGCATACACA |
| Internal bar code: | TCGTCGCGTATAGCGGAGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 63 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATTCGTGTATGTCCGCA |
| Suggested primer 2: | ATTCATTTACACGCATGCCA |