Insertion junction: LMJ.RY0402.231126_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.231126
Chromosome:chromosome 3
Location:1283726
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g150251 (1 of 80) IPR003882 - Pistil-specific extensin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTGTCCACCTTCCCCCACAGGTGCCGC
Internal bar code:CCCGTAACGCAACACTCAAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:264
LEAP-Seq percent confirming:99.7835
LEAP-Seq n confirming:922
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACGACTGGTCGCAGTTCTTT
Suggested primer 2:AGCAGCTAGCTACGAGACCG