Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.231150 |
Chromosome: | chromosome 12 |
Location: | 6246928 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g536900 | (1 of 1) IPR000104//IPR003072//IPR003495//IPR011629//IPR012336//IPR027417 - Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type // CobW/HypB/UreG domain // Cobalamin (vitamin B12) biosynthesis CobW-like, C-terminal // Thioredoxin-like fold // P-loop containing nucleoside triphosphate hydrolase; Putative metallochaperone | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGGTTGGCAGGAGCGCGGGCAGAGGTC |
Internal bar code: | CCTCCCAGCCGAGGGCAGGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 387 |
LEAP-Seq percent confirming: | 90.0217 |
LEAP-Seq n confirming: | 7867 |
LEAP-Seq n nonconfirming: | 872 |
LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAGCACCACACCTACCTGC |
Suggested primer 2: | GTTGCTTTCCCGTAGCACTC |