| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231155 |
| Chromosome: | chromosome 16 |
| Location: | 861512 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647901 | CSC8 | Chlamydomonadale-specific family C protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGTGACGAGGTCAACGTTTCACCCCTG |
| Internal bar code: | GATGTGGGCCGTGCCGGTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 90.7692 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGTTCGCGTCTAATGCGT |
| Suggested primer 2: | GATGGCTAAAGCATTCCCAA |