| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231155 |
| Chromosome: | chromosome 16 |
| Location: | 900833 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCATGTACCGCCACCCCAACCGGCTC |
| Internal bar code: | CCTCATGTATGGATTCGCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 99.8845 |
| LEAP-Seq n confirming: | 2595 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGTGGGATTGAGGATG |
| Suggested primer 2: | GATTGGTGTTGCACATCGTC |