| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231156 |
| Chromosome: | chromosome 2 |
| Location: | 6439999 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115850 | (1 of 3) PF01987 - Mitochondrial biogenesis AIM24 (AIM24) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGAACCTGGCCAACGCGCTCGTGCCA |
| Internal bar code: | CACGTGGGTTTGCGTGCCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 92.158 |
| LEAP-Seq n confirming: | 4783 |
| LEAP-Seq n nonconfirming: | 407 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCATACCCATGTTCTTG |
| Suggested primer 2: | TACTGCTCACTGCCATCAGG |