| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231172 |
| Chromosome: | chromosome 12 |
| Location: | 5889580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGGCGTTGGATGCGGGGGACCTCGCG |
| Internal bar code: | CTGGGCGACGCTGCCTCCAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 67.9426 |
| LEAP-Seq n confirming: | 5254 |
| LEAP-Seq n nonconfirming: | 2479 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGAAAACAGCCCTCAC |
| Suggested primer 2: | ACACCTGGTTTTTGTCTGCC |