| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231176 |
| Chromosome: | chromosome 9 |
| Location: | 4712592 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397031 | (1 of 6) 5.3.99.5 - Thromboxane-A synthase / Thromboxane synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCTGCTGCCCCCAGCTTATTTCCCAGC |
| Internal bar code: | TGGACGTACATCTGTGTAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 97.7528 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCGGCTCAGTAGATGC |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |