| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231185 |
| Chromosome: | chromosome 16 |
| Location: | 3666454 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688600 | XPG4 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGGCGTGGGCGTGGGGTGCGACAGGG |
| Internal bar code: | GGCACCCGGGCTGAGCGCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACTCACGTCAACCCCT |
| Suggested primer 2: | ATAAAAGAATCCCCCGCATC |