Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.231189 |
Chromosome: | chromosome 11 |
Location: | 2456539 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g475500 | 5'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTCACATGCGTCCAGTCGCTTGAGCAAG |
Internal bar code: | GGTTTGGAGACACGGGGTTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 554 |
LEAP-Seq percent confirming: | 98.8827 |
LEAP-Seq n confirming: | 354 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCTATGCTTTCCTGACTGC |
Suggested primer 2: | GGGCTTGTTTGCAACAGATT |