Insertion junction: LMJ.RY0402.231201_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.231201
Chromosome:chromosome 2
Location:3483848
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095122 FKB16H,FKB16-8,FKB11 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 25) PTHR10516 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCTACTATCACACCAATACGCACTGAG
Internal bar code:TCCGCGGCTATCACAGGGAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:595
LEAP-Seq percent confirming:97.8585
LEAP-Seq n confirming:1051
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCAACCAACATCCCAAT
Suggested primer 2:CTTGTCTCGGAGCTACAGGG