| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231203 |
| Chromosome: | chromosome 2 |
| Location: | 92370 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g073900 | (1 of 2) 1.13.11.69 - Carlactone synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACGCCTGTGCCCGCGGAGGGGCCGTTGG |
| Internal bar code: | GTCAAAGAGCGCTTGGGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCTTGTCCTTGTCCTCC |
| Suggested primer 2: | TGCACACGTTACCCTTTCAA |