| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231204 |
| Chromosome: | chromosome 14 |
| Location: | 2953928 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627726 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCTGGCATCGTGCGTATTTTCTCAAA |
| Internal bar code: | GGGGGATTGGGATCGCAATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1049 |
| LEAP-Seq percent confirming: | 99.4179 |
| LEAP-Seq n confirming: | 3416 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGTGTCCACAGTCCAC |
| Suggested primer 2: | CTTCAGCTTCATGTACGCCA |