| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231216 |
| Chromosome: | chromosome 10 |
| Location: | 4279247 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450600 | (1 of 1) PTHR10024 - SYNAPTOTAGMIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGATCATTGCTTAGTGCGTGCGCGTCGC |
| Internal bar code: | CCGGGGCTTGCCCCGGGGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 98.6441 |
| LEAP-Seq n confirming: | 873 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTCCTATCAACAAGGCG |
| Suggested primer 2: | TCTGTCACATGCCTTTGAGG |