| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231244 |
| Chromosome: | chromosome_2 |
| Location: | 608512 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077850 | FAP212 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGAACGTGGCGGTTACGGAAGCCTACAT |
| Internal bar code: | CCCTTTCGCGAAGTGCATATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 95.9091 |
| LEAP-Seq n confirming: | 633 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCAATTGCCAGGTACAGC |
| Suggested primer 2: | TAACGTGACCAAGGACACCA |