| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231250 |
| Chromosome: | chromosome 16 |
| Location: | 6218711 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675100 | CPLD53 | Conserved in the Plant Lineage and Diatoms; (1 of 3) PTHR31319:SF10 - ZINC FINGER PROTEIN CONSTANS-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATCAGCGCGTGCCGCATCTTCGTCGCT |
| Internal bar code: | GGATTAGTCACGTCCGTGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.4475 |
| LEAP-Seq n confirming: | 2700 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGAGGTGTTGCATGTTT |
| Suggested primer 2: | TATGTTACCGTTGTCCGCAA |