| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231262 |
| Chromosome: | chromosome 1 |
| Location: | 1707082 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009101 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACGTCAACCTGTGACACACACCAGAATG |
| Internal bar code: | GTGCGCGGACATGATGACATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 98.7342 |
| LEAP-Seq n confirming: | 234 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCACTCGCACAGCATTC |
| Suggested primer 2: | CCCGTTGCTGGATGTATTTT |