| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231270 |
| Chromosome: | chromosome 1 |
| Location: | 3365472 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021300 | (1 of 2) IPR016140 - Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATTTGCGGTGGCTGAGGGGTGCTACTGC |
| Internal bar code: | CGACAGGACAACGCAGGGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 98.9542 |
| LEAP-Seq n confirming: | 757 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACAACCACTCACAACCG |
| Suggested primer 2: | TTTTATTGGCGTTGTGGTGA |