| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231279 |
| Chromosome: | chromosome 6 |
| Location: | 8034043 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304250 | RLS2 | RegA/Rls-like protein 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCATTTACGTGGGGGGGGGCGACAGGT |
| Internal bar code: | TGCAGTCTGGCTCTGGCTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCCAATCATTACAGTCT |
| Suggested primer 2: | ATCGATGGTTGTGGATGGAT |