| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231289 |
| Chromosome: | chromosome 15 |
| Location: | 230352 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638101 | (1 of 1) PF03692 - Putative zinc- or iron-chelating domain (CxxCxxCC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAGTGTGCACTTCGGGCCATGTTGTAC |
| Internal bar code: | TCGTTTTGATCGCGGATGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 98.8438 |
| LEAP-Seq n confirming: | 12481 |
| LEAP-Seq n nonconfirming: | 146 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCCCAAACTAAACGG |
| Suggested primer 2: | TTGGGCTTGGTTTAGTTTGG |