Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.231315 |
Chromosome: | chromosome_1 |
Location: | 3912507 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g025450 | FAP122,DRC9 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGAGAAGAAGAAGGGCCTGTCGACGCTCA |
Internal bar code: | ATTGTCCACCGGCTTCCTCACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1122 |
LEAP-Seq percent confirming: | 98.8166 |
LEAP-Seq n confirming: | 1169 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTTCTCCTCCGCCTTCTTC |
Suggested primer 2: | GCGGAAAACATGTTGAAGGT |