| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231315 |
| Chromosome: | chromosome 1 |
| Location: | 3912507 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025450 | DRC9,FAP122 | Nexin-dynein regulatory complex 9; (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGAAGAAGAAGGGCCTGTCGACGCTCA |
| Internal bar code: | ATTGTCCACCGGCTTCCTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1122 |
| LEAP-Seq percent confirming: | 98.8166 |
| LEAP-Seq n confirming: | 1169 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCTCCTCCGCCTTCTTC |
| Suggested primer 2: | GCGGAAAACATGTTGAAGGT |