| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231323 |
| Chromosome: | chromosome 8 |
| Location: | 4946018 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385450 | (1 of 1) PTHR22769//PTHR22769:SF42 - MUTT/NUDIX HYDROLASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGAAGAGCGGGAACAAGAAGGGCCGATG |
| Internal bar code: | TACTTCTGTTTTCACCCGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 99.8404 |
| LEAP-Seq n confirming: | 1251 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACGCACACAC |
| Suggested primer 2: | GCAAAGTCCGAGTCCATCAT |