| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231342 |
| Chromosome: | chromosome 9 |
| Location: | 5722002 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401663 | (1 of 1) K14795 - ribosomal RNA-processing protein 36 (RRP36) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTGTGTCATGAGGGGTGTTCATGAACG |
| Internal bar code: | GTGGTGAATCGATTGCGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 93.913 |
| LEAP-Seq n confirming: | 216 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGGATGACAAAGGCAGC |
| Suggested primer 2: | TCCTCATCCTCATCCTCACC |