| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231360 |
| Chromosome: | chromosome 13 |
| Location: | 3394529 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587050 | ERF1 | Eukaryotic release factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGACCATGCTACGTACCGTATGTCGA |
| Internal bar code: | TTAGACAAGTGGGTTGCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.0557 |
| LEAP-Seq n confirming: | 2098 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTACCGTCACACTGGGC |
| Suggested primer 2: | TGCTATCTCATCACGCAAGG |