Insertion junction: LMJ.RY0402.231362_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.231362
Chromosome:chromosome 3
Location:2013405
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g156000 (1 of 8) IPR014710 - RmlC-like jelly roll fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTATGAGACAAGAAACCTGCGTCAGGACTG
Internal bar code:GGGGGACCAAAGGGGTGCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:578
LEAP-Seq percent confirming:99.2453
LEAP-Seq n confirming:263
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AAACGCCTACACCACACCTC
Suggested primer 2:AGCTCACCAACTCGACCACT