Insertion junction: LMJ.RY0402.231362_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.231362
Chromosome:chromosome 17
Location:6604668
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g743547 (1 of 16) IPR003439//IPR003593//IPR013525//IPR027417 - ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACGCGGCGCTTCCAGTCGCTGAGCACTC
Internal bar code:TGCTATCTGTGGCCCCATTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:504
LEAP-Seq percent confirming:99.4005
LEAP-Seq n confirming:4145
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCCCGCCATGAAGTACAC
Suggested primer 2:CCATGCACAGCTTTTTGCTA