| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231383 |
| Chromosome: | chromosome 6 |
| Location: | 1544445 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260050 | (1 of 27) IPR015947 - PUA-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGGCCAGCGCGACATGGGGTCGGCGCG |
| Internal bar code: | GAGCGGCCCACGTGACTTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 99.518 |
| LEAP-Seq n confirming: | 6194 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATTGGACTGACTGGTGG |
| Suggested primer 2: | ATTCACCTCCATCAGAACGC |