Insertion junction: LMJ.RY0402.231384_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.231384
Chromosome:chromosome 2
Location:1014016
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g080450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTGTTTCTGTCCCTGTTCTCGTGTTGT
Internal bar code:TCCGGTTGGTGTCTGCCCTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:345
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:106
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGCTCTTGCTTGATTG
Suggested primer 2:CCTGGAGCACTTCCACTACC