Insertion junction: LMJ.RY0402.231385_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.231385
Chromosome:chromosome 9
Location:3310165
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g388319 (1 of 1) PTHR13390:SF0 - UPF0554 PROTEIN C2ORF43 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCCCGGTCCGTGGCTGGTGGCTGGCAA
Internal bar code:GCTGGACCGGGTAGCCCGGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:487
LEAP-Seq percent confirming:99.2974
LEAP-Seq n confirming:424
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGATGTACGGAGATGACG
Suggested primer 2:GGCGCTGTATAGCGAAAGTC