| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231401 |
| Chromosome: | chromosome 16 |
| Location: | 1380891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652050 | ZXE3 | (1 of 1) PTHR13789//PTHR13789:SF234 - MONOOXYGENASE // SUBFAMILY NOT NAMED; Putative zeaxanthin epoxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATGGAAACCCCGTCCCGGTGTCCTGCT |
| Internal bar code: | AGCCCATAAAAGTCTAGGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 153 |
| LEAP-Seq percent confirming: | 98.0769 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGACGTGATGTGGGTTG |
| Suggested primer 2: | TCCGGCATTGTGTTTGAGTA |