| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231405 |
| Chromosome: | chromosome 14 |
| Location: | 2643063 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626200 | P4H7,PFH7,PHX18 | Prolyl 4-hydroxylase 7; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGCACCATCTAGCTTCCATGTCAGGC |
| Internal bar code: | CGCCGGAACTCCAGGCCTACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGTTCGCTGATGTTGCT |
| Suggested primer 2: | GCTGCTGTTCCATAGCATGA |