Insertion junction: LMJ.RY0402.231417_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.231417
Chromosome:chromosome 7
Location:6172379
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g356150 (1 of 1) 3.4.24.84 - Ste24 endopeptidase / Prenyl protein-specific endoprotease 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCCAGCCTGACAACGCGCTCCCCGGAC
Internal bar code:CCTGCGGAGGGGACCTAGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:172
LEAP-Seq percent confirming:97.7658
LEAP-Seq n confirming:4726
LEAP-Seq n nonconfirming:108
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAAGCTCTTTCACACTGC
Suggested primer 2:ATCACCACACGCTTACCTCC