| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231426 |
| Chromosome: | chromosome 1 |
| Location: | 2256977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012350 | (1 of 1) K02331 - DNA polymerase phi [EC:2.7.7.7] (POL5, MYBBP1A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCCTCGTCACCCTCATCCGACATGCGC |
| Internal bar code: | CGCTAAGTTACAAGCGGATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 96.6387 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAAGAGCAACACACCTGC |
| Suggested primer 2: | AAATGAGAGCTCCATGGGTG |